Slow reacting substances of anaphylaxis: Identification of leukotrienes C-1 and D from human and rat sources

• DOI: 10.1073/pnas.77.6.3710
• Publication Date: 2006-05-31T08:21:20Z
• Authors: Robert A. Lewis; K. Frank Austen; Jeffrey M. Drazen; David A. Clark; Anthony Marfat; E. J. Corey
• Keywords: Leukotriene C4

Slow reacting substance(s) of anaphylaxis (SRS-A) was isolated from both human (lung) and rat sources compared with three synthetic SRS-As known structure—leukotrienes (LTs) C-1, C-2, D. Reversed-phase liquid chromatography used as a final purification step means comparison biologically derived substances. Two major peaks SRS-A activity origin corresponded chromatographically LTC-1 LTD, respectively, had equivalent specific activities on the guinea pig ileum. With ileum, (units/pmol) for leukotrienes anaphylactic were (mean ± SEM): LTC-1, 1.93 0.13; peak I, 1.69 0.43; 6.10 1.15; II, 7.14 0.51; hu 1.90. Both LTD their natural counterparts preferential contractile peripheral airway to central airways at least 200 times more active than histamine molar basis. Leukotriene D is lung accounts almost all biological activity. It likely formed leukotriene C-1 in vivo by an enzymic process well-known γ-glutamyltransferase type.