Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells.

• DOI: 10.1073/pnas.77.7.4251
• Publication Date: 2006-05-31T08:21:40Z
• Authors: P Kavathas; F H Bach; R DeMars
• Keywords: Histocompatibility

Gamma rays from a cesium source were used to generate human lymphoblastoid cell line variants that had lost expression of all major histocompatibility complex antigens coded for by single haplotype. The was heterozygous at the glyoxalase I locus and HLA haplotypes HLA-A1, B8, DRw3, HLA-A2, B5, DRw1. We selected with anti-HLA-B8 antiserum in population cells been irradiated 300 R. incidence B8-loss 4.1 X 10(-5) on day 5 after irradiation. Analysis showed expressions GLO alleles trans B8 retained. However, additional cis-linked gene products 12 17 variants. Variants (i) HLA-B8, (ii) A1, (iii) or (iv) DRw3 allele obtained. Karyotype analysis performed eight two more alleles. Three normal appearing no. 6 chromosomes, four deletion included region coding genes short arm one chromosome, variant an inversion translocation involving 6.