L-Fucose-terminated glycoconjugates are recognized by pinocytosis receptors on macrophages.

• DOI: 10.1073/pnas.78.2.1019
• Publication Date: 2006-05-31T08:37:48Z
• Authors: V L Shepherd; Y C Lee; P H Schlesinger; P D Stahl
• Keywords: Pinocytosis; Bovine serum albumin; Serum Albumin; Mannan; Glycoconjugate

125I-Labeled L-fucose-albumin complex and rat preputial beta-glucuronidase are rapidly cleared from plasma after intravenous infusion. L-Fucose-albumin retards the clearance of whereas D-fucose-albumin is inactive. In vitro, 125I-labeled taken up into or rabbit alveolar macrophages by receptor-mediated pinocytosis. Uptake (37 degrees C) time-dependent, saturable with increasing ligand concentration (Kuptake = 4.4 X 10(-8) M), requires Ca2+. poorly up. Binding (4 Ca2+ dependent. uptake fully inhibited yeast mannan. A series neoglycoproteins, including L-fucose-albumin, were tested as inhibitors macrophages. The following order potency was observed: L-Fuc D-Man greater than GlcNAc approximately D-Glc D-Xyl much D-Gal L-Ara D-Fuc. L-Fucose-terminated oligosaccharides coupled to bovine serum albumin also block