Human alveolar macrophages produce leukotriene B4.

• DOI: 10.1073/pnas.79.24.7866
• Publication Date: 2006-05-31T09:00:38Z
• Authors: A O Fels; N A Pawlowski; E B Cramer; T K King; Z A Cohn; W A Scott
• Keywords: Leukotriene B4; Alveolar macrophage; Pulmonary alveolus

Human alveolar macrophages obtained by bronchoalveolar lavage were labeled overnight with [3H]arachidonic acid. The cells stimulated calcium ionophore A23187, and the 20:4 oxygenated metabolites released into culture medium identified reverse-phase HPLC. Leukotriene B4 was major metabolite produced these cultures. its HPLC elution time, UV spectrum, chemotactic chemokinetic activities for neutrophils. In addition, macrophage- neutrophil-derived leukotriene free acids methyl esters found to have identical retention times.