Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DC beta gene.

• DOI: 10.1073/pnas.80.23.7313
• Publication Date: 2006-05-31T09:16:22Z
• Authors: D Larhammar; J J Hyldig-Nielsen; B Servenius; G Andersson; L Rask; P A Peterson
• Keywords: Homology; Exon shuffling

We have determined the complete nucleotide sequence of a human class II histocompatibility antigen DC beta gene. The gene spans more than 7 kilobases and contains five exons corresponding to different domains polypeptide. exon-intron organization is thus analogous that alpha-chain genes, I heavy chain constant parts immunoglobulin emphasizing further evolutionary relationship among these molecules. mature polypeptide deduced from shows 93% 88% homology, respectively, sequences derived two cDNA clones other haplotypes. allelic polymorphism chains resides predominantly in first extracellular domain, whereas rest virtually constant. display high homology murine I-A Also, introns show significant homology. lack eight amino acids cytoplasmic tail, as compared DR chains. This probably due nonfunctional splice junction causing separate exon be nonexpressed.