Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2.

• DOI: 10.1073/pnas.81.14.4510
• Publication Date: 2006-05-31T05:27:08Z
• Authors: J D Fingeroth; J J Weis; T F Tedder; J L Strominger; P A Biro; D T Fearon
• Keywords: Raji cell; Lymphoblast

Identity of the Epstein-Barr virus (EBV) receptor with complement type 2 (CR2) was established in three sets experiments using monoclonal antibodies, HB-5 and anti-B2, which recognize a Mr 145,000 B-lymphocyte membrane protein that is CR2. First, rank order for binding fluoresceinated EBV to four lymphoblastoid cell lines (SB, JY, Raji, Molt-4) identical anti-B2 by analytical flow cytometry. Second, pretreatment cells followed treatment goat F(ab')2 fragments mouse IgG blocked on SB, B-lymphoblastoid line. Virus attachment not inhibited alone, second antibody rabbit anti-C3b receptor, or UPC10 (an irrelevant antibody). Third, transfer CR2 from SB A-bearing Staphylococcus aureus particles, had been absorbed, conferred them specific ability bind 125I-labeled EBV. We conclude human B lymphocytes.