Neural BC1 RNA: cDNA clones reveal nonrepetitive sequence content.

• DOI: 10.1073/pnas.84.9.2624
• Publication Date: 2006-05-31T10:34:33Z
• Authors: T M DeChiara; J Brosius
• Keywords: Small nuclear RNA

BC1 is a small developmentally regulated RNA that prevalent in nervous tissue. In order to determine if represents the transcriptional by-product of various repetitive brain identifier (ID) elements or independent transcript single few genes, we compared sequences population cDNA clones derived from vitro C-tailed RNA. Each 10 randomly selected revealed 5' domain was identical sequence ID element, followed by an internal region poly(A). 8 clones, found identical, nonrepetitive located at 3' end each molecule. An oligonucleotide 30 residues complementary this section identified only blot-hybridization analysis. Our results strongly suggest transcribed specifically gene(s) and not highly heterogeneous ID-containing polymerase III transcripts. Moreover, availability unique will facilitate studies on tissue- stage-specific gene regulation help clarifying role brain.