Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs.

• DOI: 10.1073/pnas.85.7.2171
• Publication Date: 2006-05-31T10:51:57Z
• Authors: E A Leibold; H N Munro
• Keywords: Messenger RNP

The mRNAs for the heavy and light subunits of iron-storage protein ferritin occur in cells largely as inactive ribonucleoprotein particles, which are recruited translation when iron enters cell. Cytoplasmic extracts from rat tissues hepatoma were shown by an electrophoretic separation procedure to form RNA-protein complexes involving a highly conserved sequence 5' untranslated region both heavy- light-subunit mRNAs. pattern complex formation was affected pretreatment rats or with iron. Crosslinking UV irradiation showed that contained 87-kDa interacting mRNA. We propose intracellular levels regulate synthesis causing changes specific binding