Specific translational activation by nuclear gene products occurs in the 5' untranslated leader of a yeast mitochondrial mRNA.

• DOI: 10.1073/pnas.85.8.2677
• Publication Date: 2006-05-31T10:53:09Z
• Authors: M C Costanzo; T D Fox
• Keywords: 0301 basic medicine; DNA, Recombinant; Saccharomyces cerevisiae; Cytochrome b Group; Mitochondria; Electron Transport Complex IV; Fungal Proteins; 03 medical and health sciences; Suppression, Genetic; Gene Expression Regulation; Genes; Protein Biosynthesis; Mutation; RNA, Messenger

Translation of the yeast mitochondrial mRNA encoding cytochrome c oxidase subunit III (coxIII) is specifically activated by products at least three nuclear genes, PET494, PET54, and PET122. To investigate whether target site for translational activation within 5' untranslated leader coxIII mRNA, we asked translation another protein, apo-cytochrome b, from a chimeric bearing required or Mutations in any these genes abolished b an two-thirds leader, showing that all gene are must act leader. Our data suggest wild-type cells, specific PET122 occurs action sites region 172 nucleotides upstream initiation codon.