Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae.

• DOI: 10.1073/pnas.86.14.5419
• Publication Date: 2006-05-31T10:59:30Z
• Authors: R Erdmann; M Veenhuis; D Mertens; W H Kunau
• Keywords: 0301 basic medicine; catalase; Genetic Complementation Test; Oleic Acids; Saccharomyces cerevisiae; Catalase; Microbodies; Fumarate Hydratase; Microscopy, Electron; 03 medical and health sciences; protein import machinery; Mutation; Zellweger syndrome; β-oxidation; Acetyl-CoA C-Acetyltransferase; Oleic Acid

Two mutants of Saccharomyces cerevisiae affected in peroxisomal assembly (pas mutants) have been isolated and characterized. Each strain contains a single mutation that results (i) the inability to grow on oleic acid, (ii) accumulation matrix enzymes cytosol, (iii) absence detectable peroxisomes at ultrastructural level. These lesions (pas1-1 pas2) are shown be nonallelic recessive. Crossing pas1-1 pas2 strains resulted diploid cells had regained ability acid as sole carbon source form peroxisomes. pas may provide useful tools for future studies molecular mechanisms involved assembly.