Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae.

0301 basic medicine catalase Genetic Complementation Test Oleic Acids Saccharomyces cerevisiae Catalase Microbodies Fumarate Hydratase Microscopy, Electron 03 medical and health sciences protein import machinery Mutation Zellweger syndrome β-oxidation Acetyl-CoA C-Acetyltransferase Oleic Acid
DOI: 10.1073/pnas.86.14.5419 Publication Date: 2006-05-31T10:59:30Z
ABSTRACT
Two mutants of Saccharomyces cerevisiae affected in peroxisomal assembly (pas mutants) have been isolated and characterized. Each strain contains a single mutation that results (i) the inability to grow on oleic acid, (ii) accumulation matrix enzymes cytosol, (iii) absence detectable peroxisomes at ultrastructural level. These lesions (pas1-1 pas2) are shown be nonallelic recessive. Crossing pas1-1 pas2 strains resulted diploid cells had regained ability acid as sole carbon source form peroxisomes. pas may provide useful tools for future studies molecular mechanisms involved assembly.
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