Gene VI of figwort mosaic virus (caulimovirus group) functions in posttranscriptional expression of genes on the full-length RNA transcript.

• DOI: 10.1073/pnas.86.23.9203
• Publication Date: 2006-05-31T11:09:14Z
• Authors: S Gowda; F C Wu; H B Scholthof; R J Shepherd
• Keywords: Cauliflower mosaic virus; Coding region; Chloramphenicol acetyltransferase

Experimental evidence for a molecular function gene VI of the caulimoviruses is presented. Based on experiments with figwort mosaic virus (FMV), it appears that has role in posttranscriptional expression closely packed genes (VII and I-V), which appear larger, full-length RNA transcript this virus. Gene its flanking 5'/3' signals included as separate plasmid during electroporation DNA into protoplasts Nicotiana edwardsonii shows an unusual type transactivation chloramphenicol acetyltransferase (CAT) fused at 5' end to small open reading frame (gene VII) long leader FMV genome. The level activity CAT increased up 20-fold over control plasmids when mixture. Mutagenesis coding portions pGS1 RVI, transactivating used experiments, demonstrated was probably polypeptide product responsible effect. Experiments various large, showed region VII necessary event. Clones cauliflower (CaMV) or intact were found reciprocally transactivate VII-CAT fusions (FMV) I-CAT located downstream sequences either viral