Expansion of the alpha 2-adrenergic receptor family: cloning and characterization of a human alpha 2-adrenergic receptor subtype, the gene for which is located on chromosome 2.

• DOI: 10.1073/pnas.87.13.5094
• Publication Date: 2006-05-31T11:22:10Z
• Authors: J W Lomasney; W Lorenz; L F Allen; K King; J W Regan; T L Yang-Feng; M G Caron; R J Lefkowitz
• Keywords: Alpha (finance)

Pharmacologic, biochemical, and genetic analyses have demonstrated the existence of multiple alpha 2-adrenergic receptor (alpha 2AR) subtypes. We cloned a human 2AR by using polymerase chain reaction with oligonucleotide primers homologous to conserved regions previously 2ARs, genes for which are located on chromosomes 4 (C4) 10 (C10). The deduced amino acid sequence encodes protein 450 acids whose putative topology is similar that family guanine nucleotide-binding protein-coupled receptors, but structure most closely resembles 2ARs. Competition curve analysis binding properties expressed in COS-7 cells variety adrenergic ligands demonstrates unique pharmacology. Hybridization somatic cell hybrids shows gene this chromosome 2. Northern blot various rat tissues expression liver kidney. pharmacology tissue localization suggest an subtype not identified classical pharmacological or ligand approaches.