Human macrophage scavenger receptors: primary structure, expression, and localization in atherosclerotic lesions.

• DOI: 10.1073/pnas.87.23.9133
• Publication Date: 2006-05-31T11:30:35Z
• Authors: A Matsumoto; M Naito; H Itakura; S Ikemoto; H Asaoka; I Hayakawa; H Kanamori; H Aburatani; F Takaku; H Suzuki
• Keywords: 0301 basic medicine; Base Sequence; Arteriosclerosis; Macrophages; Molecular Sequence Data; Chromosome Mapping; Gene Expression; Membrane Proteins; DNA; Polymerase Chain Reaction; Muscle, Smooth, Vascular; Cell Line; 3. Good health; 03 medical and health sciences; Animals; Humans; Cattle; Amino Acid Sequence; Cloning, Molecular; Peptides; Aorta; Chromosomes, Human, Pair 8; Gene Library

Two types of cDNAs for human macrophage scavenger receptors were cloned from a cDNA library derived from the phorbol ester-treated human monocytic cell line THP-1. The type I and type II human scavenger receptors encoded by these cDNAs are homologous (73% and 71% amino acid identity) to their previously characterized bovine counterparts and consist of six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), alpha-helical coiled-coil (IV), collagen-like (V), and a type-specific C-terminal (VI). The receptor gene is located on human chromosome 8. The human receptors expressed in CHO-K1 cells mediated endocytosis of modified low density lipoproteins. Two mRNAs, 4.0 and 3.2 kilobases, have been detected in human liver, placenta, and brain. Immunohistochemical studies using an anti-peptide antibody which recognizes human scavenger receptors indicated the presence of the scavenger receptors in the macrophages of lipid-rich atherosclerotic lesions, suggesting the involvement of scavenger receptors in atherogenesis.