Heritable major histocompatibility complex class II-associated differences in production of tumor necrosis factor alpha: relevance to genetic predisposition to systemic lupus erythematosus.

• DOI: 10.1073/pnas.87.3.1233
• Publication Date: 2006-05-31T11:33:07Z
• Authors: C O Jacob; Z Fronek; G D Lewis; M Koo; J A Hansen; H O McDevitt
• Keywords: Male; Tumor Necrosis Factor-alpha; Genes, MHC Class II; Genes, MHC Class I; Lymphocyte Activation; Pedigree; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; Reference Values; Humans; Lupus Erythematosus, Systemic; Female; Lymphocytes; Lymphotoxin-alpha; Alleles

We report on the production of tumor necrosis factor (TNF)-alpha and TNF-beta by mitogen-activated peripheral blood lymphocytes or enriched monocyte subpopulations from human leukocyte antigen (HLA)-typed healthy subjects. The results indicate that HLA-DR2- and DQw1-positive donors frequently exhibit low production of TNF-alpha, whereas DR3- and DR4-positive subjects show high levels of TNF-alpha production. No correlation between TNF-alpha levels and HLA-A, -B, and -C genotype was found. The relevance of this quantitative polymorphism to the genetic predisposition to lupus nephritis in systemic lupus erythematosus (SLE) patients was investigated. DR2, DQw1-positive SLE patients show low levels of TNF-alpha inducibility; this genotype is also associated with an increased incidence of lupus nephritis. DR3-positive SLE patients, on the other hand, are not predisposed to nephritis, and these patients have high TNF-alpha production. DR4 haplotype is associated with high TNF-alpha inducibility and is negatively correlated with lupus nephritis. These data may help explain the strong association between HLA-DR2, DQw1 in SLE patients and their susceptibility to nephritis.