Protection against lethal Sendai virus infection by in vivo priming of virus-specific cytotoxic T lymphocytes with a free synthetic peptide.

• DOI: 10.1073/pnas.88.6.2283
• Publication Date: 2006-05-31T11:56:31Z
• Authors: W M Kast; L Roux; J Curren; H J Blom; A C Voordouw; R H Meloen; D Kolakofsky; C J Melief
• Keywords: Sendai virus; CTL*; Priming (agriculture)

The only peptide of Sendai virus that is recognized by cytotoxic T lymphocytes (CTL) in B6 mice was found with (i) the use recombinant vaccinia constructs containing separate genes and (ii) a set overlapping peptides completely spanning identified nucleoprotein (NP) gene product. This immunodominant NP virus-specific CTL are known to have therapeutic effects vivo. By subcutaneous immunization, this induced peptide-specific memory responses Most importantly, had been immunized were protected against lethal dose, indicating viral can be used as antiviral T-cell vaccines. induction free immunization potentially has wide applicability biology medicine, including protection infectious disease.