A metastatic nude-mouse model of human pancreatic cancer constructed orthotopically with histologically intact patient specimens.

• DOI: 10.1073/pnas.89.12.5645
• Publication Date: 2006-05-31T12:02:18Z
• Authors: X Fu; F Guadagni; R M Hoffman
• Keywords: Carcinoembryonic antigen; Pancreatic tumor; Nude mouse

Pancreatic cancer is one of the most intractable and least understood all human cancers. cancers fourth-leading cause cancer-related mortality in United States with less than 2% patients surviving for 5 yr. In an effort to help develop more effective treatment modalities pancreatic improve detection, we report animal model individual pancreatic-cancer patients. The involves orthotopic transplantation histologically intact specimens nude-mouse pancreas, which can result models that resemble clinical picture including (i) extensive local tumor growth, (ii) extension locally growing stomach duodenum, (iii) metastases liver regional lymph nodes, (iv) distant adrenal gland, diaphragm, mediastinal nodes. a series five patient cases, 100% take rate has been demonstrated, 17 mice transplanted, 15 supported growth. Immunohistochemical analysis antigenic phenotype transplanted tumors showed similar pattern expression two different tumor-associated antigens, such as glycoprotein 72 carcinoembryonic antigen when compared original surgical biopsy, suggesting similarity between two. This should, therefore, prove valuable evaluation patients, well experimental this disease.