De novo generation of neuronal cells from the adult mouse brain.

• DOI: 10.1073/pnas.89.18.8591
• Publication Date: 2006-05-31T12:08:01Z
• Authors: L J Richards; T J Kilpatrick; P F Bartlett
• Keywords: Neurofilament; Thymidine; Chemically defined medium

Cells of neuronal morphology, expressing the 150- and 200-kDa neurofilament proteins, were generated in vitro from populations neural cells dissociated adult (greater than 60-day-old) mouse brain. Most these neurons arose dividing precursors, as demonstrated by incorporation [3H]thymidine during culture period autoradiography. Neuronal production was optimal under conditions which precursors initially stimulated with basic fibroblast growth factor then exposed to medium conditioned an astrocytic cell line, Ast-1, serum-free medium. Few, if any, control cultures or kept serum factor. These results suggest that exist mammalian brain, but they require discrete epigenetic signals for their proliferation differentiation.