Growth inhibition by transforming growth factor beta (TGF-beta) type I is restored in TGF-beta-resistant hepatoma cells after expression of TGF-beta receptor type II cDNA.

• DOI: 10.1073/pnas.90.11.5359
• Publication Date: 2006-05-31T12:23:57Z
• Authors: M Inagaki; A Moustakas; H Y Lin; H F Lodish; B I Carr
• Keywords: ACVRL1; TGF beta 1; BETA (programming language); R-SMAD

The growth of human hepatoma Hep 3B cells is potently inhibited by TGF-beta 1 (ID50 = 0.2 ng/ml, 8 pM). A mutant cell line was derived that not in at 5 ng/ml (200 pM) and lacked receptor type II (TGF-beta RII) gene. Transfection the cloned cDNA for RII to this restored expression as well inhibition 1. In both wild-type stably transfected with cDNA, coimmunoprecipitated I presence ligand. These experiments provide direct evidence role inhibitory effect on suggest acts means a heteromeric surface complex I.