Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination.

• DOI: 10.1073/pnas.90.13.6100
• Publication Date: 2006-05-31T12:25:50Z
• Authors: D P Silver; E Spanopoulou; R C Mulligan; D Baltimore
• Keywords: FLP-FRT recombination; Homology; V(D)J recombination; RAG2; Recombination signal sequences

As a probe of whether RAG-1 and RAG-2 gene products activate other genes or form part the recombinase itself, certain mutants RAG were assayed for their ability to variable-diversity-joining region [V(D)J] recombination in plasmid substrate fibroblasts. The results indicate that N-terminal one-third RAG-1, including zinc-finger-like domain, an acidic domain are dispensable activating V(D)J fibroblast, although they contribute quantitatively. In contrast, deletion C-terminal segment which has homology topoisomerase-like protein from yeast, abolished activation. These do not support hypothesis transcription factors suggest possibility parts machinery.