Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.

• DOI: 10.1073/pnas.91.23.11207
• Publication Date: 2006-05-31T12:59:11Z
• Authors: F B Gao; C C Carson; T Levine; J D Keene
• Keywords: AU-rich element

Hel-N1, a human RNA-binding protein, shares significant homology with Drosophila protein ELAV, which is essential for fly neuronal development. Hel-N1 has been shown to bind in vitro 3' untranslated regions of mRNAs encoding c-myc, c-fos, granulocyte/macrophage colony-stimulating factor, and transcriptional repressor, Id. We report that related form, Hel-N2, are expressed medulloblastoma cells, but their ratio differs significantly from adult brain fetal brain. Selection RNA targets randomized combinatorial libraries yielded (A+U)-rich consensus sequences both Hel-N2. As means identify cellular these proteins, we devised shape representing naturally derived were able select structurally subset transcripts bound Hel-N1. Approximately 10% the proteins encoded by identifiable data bases most implicated cell growth regulation. This approach provides gain access novel genes various types partitioning containing common sequence elements using proteins.