Cytidine methylation of regulatory sequences near the pi-class glutathione S-transferase gene accompanies human prostatic carcinogenesis.

• DOI: 10.1073/pnas.91.24.11733
• Publication Date: 2006-05-31T12:59:41Z
• Authors: W H Lee; R A Morton; J I Epstein; J D Brooks; P A Campbell; G S Bova; W S Hsieh; W B Isaacs; W G Nelson
• Keywords: GSTP1; Glutathione S-transferase; CpG site

Hypermethylation of regulatory sequences at the locus pi-class glutathione S-transferase gene GSTP1 was detected in 20 human prostatic carcinoma tissue specimens studied but not normal tissues or exhibiting benign hyperplasia. In addition, a striking decrease expression found to accompany carcinogenesis. Immunohistochemical staining with anti-GSTP1 antibodies failed detect enzyme 88 91 carcinomas analyzed. vitro, limited cancer cell lines containing alleles hypomethylated promoter sequences; line only hypermethylated did express mRNA polypeptides. Methylation cytidine nucleotides constitutes most common genomic alteration yet described for prostate cancer.