Human immunodeficiency virus type 1 Nef associateswith a cellular serine kinase in T lymphocytes.

• DOI: 10.1073/pnas.91.4.1539
• Publication Date: 2006-05-31T13:03:29Z
• Authors: E T Sawai; A Baur; H Struble; B M Peterlin; J A Levy; C Cheng-Mayer
• Keywords: MAP2K7; c-Raf; Cyclin-dependent kinase 9

With T-cell lines constitutively expressing Nef from the SF2 strain of human immunodeficiency virus type 1 (HIV-1SF2) in form a hybrid CD8-Nef fusion protein or chronically infected with HIV-1SF2, cellular serine kinase was found that specifically associates Nef. Proteins 62 kDa and 72 kDa, which coimmunoprecipitated Nef, were phosphorylated vitro assays. This Nef-associated activity not blocked by inhibitors C A lost when truncated at amino acid 94 99. These findings present evidence is associated expressed T lymphocytes.