Biochemical evidence for a complex involving dihydropyridine receptor and ryanodine receptor in triad junctions of skeletal muscle.

• DOI: 10.1073/pnas.91.6.2270
• Publication Date: 2006-05-31T13:05:57Z
• Authors: I Marty; M Robert; M Villaz; K De Jongh; Y Lai; W A Catterall; M Ronjat
• Keywords: Immunoprecipitation

Membrane vesicles enriched in both ryanodine receptor and dihydropyridine were obtained from rabbit skeletal muscle solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Analysis of the sedimentation behavior proteins showed existence a population alpha 1 subunits which cosedimented receptor. Solubilized immunoprecipitated antibodies directed against either or 1, 2, beta Immunoprecipitated identified by Western blot analysis specific labeling [3H]ryanodine [3H]PN200-110. Immunoprecipitation led to coimmunoprecipitation Conversely, immunoprecipitation an immune complex containing receptors, but these unable precipitate purified These results demonstrate that are present triad membrane preparation may play important role excitation-contraction coupling.