C3G, a guanine nucleotide-releasing protein expressed ubiquitously, binds to the Src homology 3 domains of CRK and GRB2/ASH proteins.

• DOI: 10.1073/pnas.91.8.3443
• Publication Date: 2006-05-31T13:08:01Z
• Authors: S Tanaka; T Morishita; Y Hashimoto; S Hattori; S Nakamura; M Shibuya; K Matuoka; T Takenawa; T Kurata; K Nagashima
• Keywords: Adapter molecule crk

CRK protein, together with GRB2/ASH and Nck proteins, belongs to the adaptor-type Src homology (SH)2-containing molecules, which transduce signals from tyrosine kinases. Here another guanine nucleotide-releasing protein (GNRP), C3G, has been identified as a SH3-binding protein. The nucleotide sequence of 4.1-kb C3G cDNA contains 3.2-kb open reading frame encoding 121-kDa antibodies against have shown detect 130-140 kDa. carboxyl terminus peptide homologous GNRPs for Ras, expression this region suppresses loss CDC25 function in yeast Saccharomyces cerevisiae. expressed Escherichia coli binds proteins. Mutational analysis assigns SH3 binding 50-amino acid containing proline-rich sequence. mRNAs both proteins are ubiquitously human adult fetal tissues. results these studies suggest that complex or may kinases Ras number different