Pluripotent hematopoietic stem cells contain high levels of mRNA for c-kit, GATA-2, p45 NF-E2, and c-myb and low levels or no mRNA for c-fms and the receptors for granulocyte colony-stimulating factor and interleukins 5 and 7.

• DOI: 10.1073/pnas.92.10.4601
• Publication Date: 2006-05-31T13:09:58Z
• Authors: D Orlic; S Anderson; L G Biesecker; B P Sorrentino; D M Bodine
• Keywords: Interleukin 3

Pluripotent hematopoietic stem cells (PHSCs) were highly enriched from mouse bone marrow by counterflow centrifugal elutriation, lineage subtraction, and fluorescence-activated cell sorting based on high c-kit receptor expression (c-kitBR). We used reverse transcriptase polymerase chain reaction to assay the c-kitBR subset subsets expressing low (c-kitDULL) no (c-kitNEG) for of mRNA encoding growth factor receptors transcription factors. The had approximately 3.5-fold more than unfractionated cells. c-kitDULL 47-58% found in c-kitNEG 4-9% present By comparing levels (enriched PHSCs) with those marrow, we demonstrated that contained or undetectable c-fms, granulocyte colony-stimulating receptor, interleukin 5 (IL-5R), IL-7R. These same moderate erythropoietin IL-3R subunits alpha (SUT-1), AIC-2A, AIC-2B, IL-6R its partner gp-130, GATA-1 factors GATA-2, p45 NF-E2, c-myb. conclude these findings PHSCs are programmed interact factor, IL-3, IL-6 but not macrophage factor. also indicate c-myb activities may be involved PHSC maintenance proliferation.