A role for Rho in Ras transformation.

• DOI: 10.1073/pnas.92.25.11781
• Publication Date: 2006-05-31T13:24:38Z
• Authors: R G Qiu; J Chen; F McCormick; M Symons
• Keywords: Stress fiber; 3T3 cells; Malignant Transformation; Small GTPase

The small GTP-binding proteins Rac and Rho are key elements in the signal-transduction pathways respectively controlling formation of lamellipodia stress fibers induced by growth factors or oncogenic Ras. We recently reported that function is necessary for Ras transformation expression constitutively activated Rac1 sufficient to cause malignant transformation. now show that, although V14-RhoA Rat 1 fibroblasts does not on its own, it strongly cooperates with active RafCAAX focus-formation assays NIH 3T3 cells. Furthermore, dominant-negative N19-RhoA inhibits focus V12-H-Ras cells, stable coexpression Rat1 reverts Interestingly, fiber inhibited lines restored N19-RhoA. conclude drives at least two separate pathways, one induces another important