Recognition of oxidatively damaged and apoptotic cells by an oxidized low density lipoprotein receptor on mouse peritoneal macrophages: role of membrane phosphatidylserine.

• DOI: 10.1073/pnas.92.5.1396
• Publication Date: 2006-06-02T00:59:26Z
• Authors: G R Sambrano; D Steinberg
• Keywords: CD36; Scavenger Receptor; Cell surface receptor; Phospholipid scramblase

We recently reported that oxidized low density lipoprotein (OxLDL), but not acetyl LDL (AcLDL), inhibited the binding and phagocytosis of nonopsonized, oxidatively damaged red blood cells (OxRBCs) by mouse peritoneal macrophages, implying involvement a "scavenger receptor" other than AcLDL receptor. Numerous studies establish loss plasma membrane phospholipid asymmetry, which increases phosphatidylserine expression on outer leaflet membrane, can play key role in macrophage recognition apoptotic cells. report here this is part attributable to same receptor recognizes OxLDL. As described an accompanying paper, protein 94-97 kDa. Phosphatidylserine liposomes show strong ligand 94- 97-kDa OxLDL AcLDL. Inhibition RBC translocase incubation with sodium vanadate caused progressive increase appearance cell surface parallel these RBCs was Finally, also sickled thymocytes macrophages. However, latter incomplete (approximately 50%), suggesting receptors are involved. suggest plays significant