DNA analysis and diagnostics on oligonucleotide microchips.

• DOI: 10.1073/pnas.93.10.4913
• Publication Date: 2002-07-26T14:34:16Z
• Authors: G Yershov; V Barsky; A Belgovskiy; E Kirillov; E Kreindlin; I Ivanov; S Parinov; D Guschin; A Drobishev; S Dubiley; A Mirzabekov
• Keywords: Hybridization probe; DNA–DNA hybridization

We present a further development in the technology of sequencing by hybridization to oligonucleotide microchips (SHOM) and its application diagnostics for genetic diseases. A robot has been constructed manufacture "microchips." The microchip is an array oligonucleotides immobilized into gel elements fixed on glass plate. Hybridization with fluorescently labeled DNA was monitored real time simultaneously all two-wavelength fluorescent microscope equipped charge-coupled device camera. SHOM used detect beta-thalassemia mutations patients hybridizing PCR-amplified microchips. contiguous stacking technique applied detection mutations; it can simplify medical enhance reliability. use multicolor monitoring suggested large-scale gene polymorphism studies. Other applications are discussed.