Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90.
Protein Folding
0303 health sciences
Cell-Free System
Protein Conformation
Lactams, Macrocyclic
Quinones
Saccharomyces cerevisiae
Genes, p53
Precipitin Tests
Recombinant Proteins
3. Good health
03 medical and health sciences
Protein Biosynthesis
Benzoquinones
Animals
Point Mutation
HSP90 Heat-Shock Proteins
Rabbits
Tumor Suppressor Protein p53
Triticum
Molecular Chaperones
DOI:
10.1073/pnas.93.16.8379
Publication Date:
2002-07-26T14:34:16Z
AUTHORS (4)
ABSTRACT
The p53 mutant, 143ala, was translated in vitro in either rabbit reticulocyte lysate (RRL) or wheat germ extract (WGE). In RRL, p53-143ala protein of both mutant and wild-type conformation, as detected immunologically with conformation-specific antibodies, was translated. The chaperone protein HSP90, present in RRL, was found to coprecipitate only with the mutated conformation of p53. Geldanamycin, shown previously to bind to HSP90 and destabilize its association with other proteins, decreased the amount of immunologically detectable mutated p53 and increased the amount of detectable wild-type protein, without affecting the total translation of p53. When translated in WGE, known to contain functionally deficient HSP90, p53-143ala produced p53 protein, which was not recognized by a mutated conformation-specific antibody. In contrast, the synthesis of conformationally detectable wild-type p53 in this system was not compromised. Reconstitution of HSP90 function in WGE permitted synthesis of conformationally detectable mutated p53, and this was abrogated by geldanamycin. Finally, when p53-143ala was stably tansfected into yeast engineered to be defective for HSP90 function, conformational recognition of mutated p53 was impaired. When stable transfectants of p53-143ala were prepared in yeast expressing wild-type HSP90, conformational recognition of mutated p53 was antagonized by macbecin I, a geldanamycin analog also known to bind HSP90. Taken together, these data demonstrate a role for HSP90 in the achievement and/or stabilization of the mutated conformation of p53-143ala. Furthermore, we show that the mutated conformation of p53 can be pharmacologically antagonized by drugs targeting HSP90.
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