Altered surfactant function and structure in SP-A gene targeted mice.

• DOI: 10.1073/pnas.93.18.9594
• Publication Date: 2002-07-26T14:43:20Z
• Authors: T R Korfhagen; M D Bruno; G F Ross; K M Huelsman; M Ikegami; A H Jobe; S E Wert; B R Stripp; R E Morris; S W Glasser; C J Bachurski; H S Iwamoto; J A Whitsett
• Keywords: Surfactant protein C; Gene targeting; Knockout mouse

The surfactant protein A (SP-A) gene was disrupted by homologous recombination in embryonic stem cells that were used to generate homozygous SP-A-deficient mice. SP-A mRNA and not detectable the lungs of SP-A(-/-) mice, perinatal survival mice altered compared with wild-type Lung morphology, proteins B-D, lung tissue, alveolar phospholipid pool sizes composition, compliance unaltered. At highest concentration tested, from produced same surface tension as (+/+) lower concentrations, minimum tensions higher for ultrastructural level, type II cell morphology SP-A(+/+) (-/-) While unperturbed, tubular myelin figures decreased null mutation murine interferes formation without detectably altering postnatal or pulmonary function.