A role for the Msx-1 homeodomain in transcriptional regulation: residues in the N-terminal arm mediate TATA binding protein interaction and transcriptional repression.

• DOI: 10.1073/pnas.93.5.1764
• Publication Date: 2002-07-26T14:34:16Z
• Authors: H Zhang; K M Catron; C Abate-Shen
• Keywords: Transcription; TATA-binding protein; TATA box

In a previous study we showed that the murine homeodomain protein Msx-1 is potent transcriptional repressor and this activity independent of its DNA binding function. The implication these findings repression by mediated through association with certain factors rather than interaction recognition sites, which prompted investigation relevant factors. Here show interacts directly TATA (TBP) but not several other general transcription This homeodomain, specifically residues in N-terminal arm. These same arm are required for Msx-1, suggesting functional relationship between TBP repression. further supported observation addition excess blocks action vitro assays. Finally, separable from both repression, shows activities distinct. Therefore, define role particularly protein-protein implicate more complex overall homeodomains regulation.