RAB22 and RAB163/mouse BRCA2: Proteins that specifically interact with the RAD51 protein

• DOI: 10.1073/pnas.94.13.6927
• Publication Date: 2002-07-26T14:31:39Z
• Authors: Ryushin Mizuta; Janine M. LaSalle; Hwei-Ling Cheng; Akira Shinohara; Hideyuki Ogawa; Neal Copeland; Nancy A. Jenkins; Marc Lalande; Frederick W. Alt
• Keywords: BRCA2 Protein; Recombination, Genetic; 0301 basic medicine; DNA, Complementary; Saccharomyces cerevisiae Proteins; DNA Repair; Molecular Sequence Data; Chromosome Mapping; Nuclear Proteins; RNA-Binding Proteins; Saccharomyces cerevisiae; Neoplasm Proteins; 3. Good health; DNA-Binding Proteins; Mice; 03 medical and health sciences; Animals; Humans; Amino Acid Sequence; Rad51 Recombinase; Cloning, Molecular; Carrier Proteins; Transcription Factors

The human RAD51 protein is a homologue of the bacteria RecA and yeast RAD51 proteins that are involved in homologous recombination and DNA repair. RAD51 interacts with proteins involved in recombination and also with tumor suppressor proteins p53 and breast cancer susceptibility gene 1 (BRCA1). We have used the yeast two-hybrid system to clone murine cDNA sequences that encode two RAD51-associated molecules, RAB22 and RAB163. RAB163 encodes the C-terminal portion of mouse BRCA2, the homologue of the second breast cancer susceptibility gene protein in humans, demonstrating an in vitro association between RAD51 and BRCA2. RAB22 is a novel gene product that also interacts with RAD51 in vitro . To detect RAD51 interactions in vivo , we developed a transient nuclear focus assay that was used to demonstrate a complete colocalization of RAB22 with RAD51 in large nuclear foci.