rRNA-like sequences occur in diverse primary transcripts: Implications for the control of gene expression

• DOI: 10.1073/pnas.94.2.422
• Publication Date: 2002-07-26T14:44:19Z
• Authors: Vincent P. Mauro; Gerald M. Edelman
• Keywords: 0301 basic medicine; Amanitins; Base Sequence; Transcription, Genetic; Molecular Sequence Data; Blotting, Northern; Mice; 03 medical and health sciences; Gene Expression Regulation; RNA, Ribosomal; Sequence Homology, Nucleic Acid; Animals; Humans; Cattle; RNA Polymerase II; RNA, Messenger; Chickens; Sequence Alignment; Repetitive Sequences, Nucleic Acid

Many eukaryotic mRNAs contain sequences that resemble segments of 28S and 18S rRNAs, these rRNA-like are present in both the sense antisense orientations. Some similar to highly conserved regions whereas others have sequence similarities expansion segments. In particular, four found several hundred different genes, location within various genes is not random. One preferentially located protein coding immediately upstream termination codon a number genes. Northern blot analysis poly(A) + RNA from vertebrates (chicken, cattle, rat, mouse, human) revealed large discrete molecules hybridize at high stringency cloned probes prepared or rRNA were match those mRNAs. Inhibition polymerase II activity, which prevents synthesis most mRNAs, abolished hybridization probes. We consider hypotheses may spread throughout genomes their presence primary transcripts differentially affect gene expression.