Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network

• DOI: 10.1073/pnas.94.26.14461
• Publication Date: 2002-07-26T14:31:39Z
• Authors: Manuel Muñiz; María Esther Martín; Josefina Hidalgo; Angel Velasco
• Keywords: Vesicular Transport Proteins; Budding

We have examined the role played by protein kinase A (PKA) in vesicle-mediated transport from trans-Golgi network (TGN) to cell surface. In vivo this step was inhibited inhibitors of PKA catalytic subunits (C-PKA) such as compound known H89 and a myristoylated form inhibitory peptide sequence contained thermostable inhibitor. Inhibition occurred at an early stage during transfer vesicular stomatitis virus G glycoprotein TGN Reversal inhibition correlated with transient increase number free coated vesicles Golgi area. Vesicle budding studied vitro using virus-infected, permeabilized cells. Addition assay C-PKA stimulated vesicle release while it suppressed peptide, H89, antibody against C-PKA. Furthermore, decreased when PKA-depleted cytosol used restored addition These results indicate regulatory for activity production constitutive TGN.