Increased fluid secretion after adenoviral-mediated transfer of the aquaporin-1 cDNA to irradiated rat salivary glands

• DOI: 10.1073/pnas.94.7.3268
• Publication Date: 2002-07-26T14:43:20Z
• Authors: Christine Delporte; Brian C. O’Connell; Xinjun He; Henry E. Lancaster; Anne C. O’Connell; Peter Agre; Bruce J. Baum
• Keywords: Ductal cells; Aquaporin 1; Acinar cell; Epithelial polarity

A replication-deficient, recombinant adenovirus encoding human aquaporin-1 (hAQP1), the archetypal water channel, was constructed. This virus, AdhAQP1, directed hAQP1 expression in several epithelial cell lines vitro . In polarized MDCK monolayers, localized apical and basolateral plasma membranes. Fluid movement across monolayers infected by AdhAQP1 response to an osmotic gradient ≈4-fold that seen with uninfected or a control virus. When administered rat submandibular glands retrograde ductal instillation, significant observed Western blot analysis crude membranes immunohistochemical staining both acinar cells. Three four months after exposure single radiation dose (17.5 21 Gy, respectively), administration led two- threefold increase salivary secretion compared from These results suggest gene transfer may have potential as unique approach for treatment of postradiation hypofunction.