Carcinogens induce reversion of the mouse pink-eyed unstable mutation

• DOI: 10.1073/pnas.94.9.4576
• Publication Date: 2002-07-26T14:31:39Z
• Authors: Robert H. Schiestl; Jiri Aubrecht; Fathia Khogali; Nicholas Carls
• Keywords: Reversion; Ethyl methanesulfonate; Methyl methanesulfonate

Deletions and other genome rearrangements are associated with carcinogenesis inheritable diseases. The pink-eyed unstable ( p un ) mutation in the mouse is caused by duplication of a 70-kb internal fragment gene. Spontaneous reversion events homozygous / mice occur through deletion duplicated sequence. Reversion premelanocytes embryo detected as black spots on gray fur offspring were inducible carcinogen x-rays, ethyl methanesulfonate, methyl nitrosourea, benzo[ ]pyrene, trichloroethylene, benzene, sodium arsenate. latter three carcinogens not detectable several vitro or vivo mutagenesis assays. We studied molecular mechanism carcinogen-induced cDNA analysis using reverse transcriptase–PCR method identified induced deletions. DNA assays may be sensitive indicators for exposure.