Human carbonic anhydrase XII: cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers

• DOI: 10.1073/pnas.95.13.7608
• Publication Date: 2002-07-26T14:42:40Z
• Authors: Özlem Türeci; Ugur Sahin; Evi Vollmar; Stefan Siemer; Elisabeth Göttert; Gerhard Seitz; Anna-Kaisa Parkkila; Gul N. Shah; Jeffrey H. Grubb; Michael Pfreundschuh; William S. Sly
• Keywords: Carbonic anhydrase II

We report the cloning and characterization of a tumor-associated carbonic anhydrase (CA) that was identified in human renal cell carcinoma (RCC) by serological expression screening with autologous antibodies. The cDNA sequence predicts 354-amino acid polypeptide molecular mass 39,448 Da has features type I membrane protein. predicted includes 29-amino signal sequence, 261-amino CA domain, an additional short extracellular segment, 26-amino hydrophobic transmembrane hydrophilic C-terminal cytoplasmic tail 29 amino acids contains two potential phosphorylation sites. domain shows 30–42% homology known CAs, all three Zn-binding histidine residues found active sites for asparagine glycosylation. When expressed COS cells, produced 43- to 44-kDa protein membranes had around one-sixth activity from cells transfected same vector expressing bovine IV. have designated this XII. Northern blot analysis normal tissues demonstrated 4.5-kb transcript only kidney intestine. However, 10% patients RCC, XII at much higher levels RCC than surrounding tissue. gene mapped using fluorescence situ hybridization 15q22. is second catalytically reported be overexpressed certain cancers. Its relationship oncogenesis its as clinically useful tumor marker clearly merit further investigation.