Identification of a large Myc-binding protein that contains RCC1-like repeats

• DOI: 10.1073/pnas.95.16.9172
• Publication Date: 2002-07-26T14:39:15Z
• Authors: Qingbin Guo; Jingwu Xie; Chi V. Dang; Edison T. Liu; J. Michael Bishop
• Keywords: Transcriptional Activation; 0301 basic medicine; 0303 health sciences; Chromosomes, Human, Pair 13; Sequence Homology, Amino Acid; Ubiquitin-Protein Ligases; Molecular Sequence Data; Brain; Chromosome Mapping; Nuclear Proteins; Cell Cycle Proteins; Thymus Gland; Burkitt Lymphoma; Mixed Function Oxygenases; DNA-Binding Proteins; Proto-Oncogene Proteins c-myc; 03 medical and health sciences; Guanine Nucleotide Exchange Factors; Humans; Amino Acid Sequence; Carrier Proteins; Adaptor Proteins, Signal Transducing

The protooncogene MYC plays an important role in the regulation of cellular proliferation, differentiation, and apoptosis has been implicated a variety human tumors. closely related MYCN encode highly conserved nuclear phosphoproteins (Myc NMyc) that apparently function as transcription factors cell. We have identified large protein interacts directly with transcriptional activating domain Myc (designated “protein associated Myc” or Pam). Pam contains extended amino acid sequence similarities to known regulator chromosome condensation (RCC1), which may play chromatin. gene encoding ( PAM ) is expressed all tissue examined, but expression exceptionally abundant brain thymus. binds specifically Myc, not NMyc. region required for binding includes essential frequently mutated Burkitt’s lymphomas. located within 300-kb on 13q22.