Enhanced T cell engraftment after retroviral delivery of an antiviral gene in HIV-infected individuals

• DOI: 10.1073/pnas.95.3.1201
• Publication Date: 2002-07-26T14:40:11Z
• Authors: Udaykumar Ranga; Clive Woffendin; Sunita Verma; Ling Xu; Carl H. June; D. Keith Bishop; Gary J. Nabel
• Keywords: Group-specific antigen

Intracellular expression of gene products that inhibit viral replication have the potential to complement current antiviral approaches treatment AIDS. We previously shown a mutant inhibitory form an essential protein, Rev M10, prolongs survival T cells transduced with nonviral vector in HIV-infected individuals. Because these gene-modified were not observed patients beyond 8 weeks, efforts made improve duration engraftment. In this study, we used retroviral delivery M10 CD4 + and analyzed relevant immune responses pilot study three HIV-seropositive patients. DNA RNA PCR analyses revealed vectors survived expressed recombinant for significantly longer time periods than those negative control all Immune detected either or Moloney murine leukemia virus gp70 envelope protein. M10-transduced average 6 months after transfer compared ≈3 weeks reported delivery. These findings suggest may potentially contribute reconstitution AIDS could provide more effective prolong HIV infection.