Generation of CD8 suppressor factor and β chemokines, induced by xenogeneic immunization, in the prevention of simian immunodeficiency virus infection in macaques

• DOI: 10.1073/pnas.95.9.5223
• Publication Date: 2002-07-26T14:42:40Z
• Authors: Yufei Wang; Louisa Tao; Elaine Mitchell; Willy M. J. M. Bo...; Carl Doyle; Christopher A. Br...; Lesley A. Bergmeier; Charles G. Kelly; Jonathan L. Heeney; Thomas Lehner
• Keywords: Simian immunodeficiency virus; Macrophage inflammatory protein

Previous xenogeneic immunization experiments in rhesus macaques with simian immunodeficiency virus (SIV) grown human CD4(+) T cells consistently elicited protection from challenge live SIV. However, the mechanism of has not been established. We present evidence that induced significant CD8 suppressor factor, RANTES (regulated upon activation, normal cell expressed and secreted), macrophage inflammatory protein (MIP) 1alpha, MIP-1beta (P < 0.001 - P 0.02). The concentrations these increased significantly protected as compared infected 0.001). Xenogeneic stimulation vitro also up-regulated factors (SF; 0.001) beta chemokines which were neutralized by antibodies to 3 chemokines. Recombinant RANTES, MIP-1alpha bind CCR5, suppressed SIV replication a dose-dependent manner, being more effective than other two results suggest induces CD8-suppressor may block CCR5 receptors prevent binding fusion cells.