A bioluminescence resonance energy transfer (BRET) system: Application to interacting circadian clock proteins

• DOI: 10.1073/pnas.96.1.151
• Publication Date: 2002-07-26T14:41:48Z
• Authors: Yao Xu; David W. Piston; Carl Hirschie Joh...
• Keywords: Luciferases

We describe a method for assaying protein interactions that offers some attractive advantages over previous assays. This method, called bioluminescence resonance energy transfer (BRET), uses bioluminescent luciferase is genetically fused to one candidate protein, and green fluorescent mutant another of interest. Interactions between the two fusion proteins can bring close enough occur, thus changing color emission. By using encoded by circadian (daily) clock genes from cyanobacteria, we use BRET technique demonstrate KaiB interacts form homodimers. should be particularly useful testing within native cells, especially with integral membrane or targeted specific organelles.