Making artificial antibodies: A format for phage display of combinatorial heterodimeric arrays

• DOI: 10.1073/pnas.96.11.6025
• Publication Date: 2002-07-26T14:40:52Z
• Authors: Changshou Gao; Shenlan Mao; Chih-Hung L. Lo; Peter Wirsching; Richard A. Lerner; Kim D. Janda
• Keywords: Phagemid; Protein Engineering; Filamentous bacteriophage

The gene VII protein (pVII) and IX (pIX) are associated closely on the surface of filamentous bacteriophage that is opposite end harboring widely exploited pIII protein. We developed a phagemid format wherein antibody heavy- light-chain variable regions were fused to amino termini pVII pIX, respectively. Significantly, fusion proteins interacted form functional Fv-binding domain phage surface. Our approach will be applicable display generic peptide libraries can combinatorial heterodimeric arrays. Consequently, it represents first step toward artificial antibodies selection novel biological activities.