An inverse relationship between T cell receptor affinity and antigen dose during CD4+T cell responsesin vivoandin vitro

• DOI: 10.1073/pnas.96.17.9781
• Publication Date: 2002-07-26T14:35:07Z
• Authors: William Rees; Jeremy Bender; T. Kent Teague; Ross M. Kedl; Frances Crawford; Philippa Marrack; John Kappler
• Keywords: CD4-Positive T-Lymphocytes; 0301 basic medicine; Hybridomas; Histocompatibility Antigens Class II; Immunization, Secondary; Receptors, Antigen, T-Cell; Flow Cytometry; 3. Good health; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Hyaluronan Receptors; Animals

Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity T cell hybridomas. A small, expanded population of cells detected one these in peptide-immunized C57BL/10 mice persisted for several months. This further on secondary immunization. Equating the extent binding this reagent receptor affinity, we saw little correlation immunizing peptide dose affinity at peak primary response. However, there was an inverse relation between apparent that present months after a response or stimulation either vivo vitro .