Distribution of human herpesvirus-8 latently infected cells in Kaposi’s sarcoma, multicentric Castleman’s disease, and primary effusion lymphoma

• DOI: 10.1073/pnas.96.8.4546
• Publication Date: 2002-07-26T14:39:15Z
• Authors: Nicolas Dupin; Cyril Fisher; Paul Kellam; Sam Ariad; Micheline Tulliez; Nathalie Franck; Eric van Marck; Dominique Salmon; Isabelle Gorin; Jean-Paul Escande; Robin A. Weiss; Kari Alitalo; Chris Boshoff
• Keywords: Primary effusion lymphoma; Kaposi's sarcoma; Kaposi's sarcoma-associated herpesvirus

Human herpesvirus 8 (HHV-8, also called KSHV) is linked to the etiopathogenesis of Kaposi’s sarcoma (KS), multicentric Castleman’s disease (MCD), and primary effusion lymphoma (PEL). The universal presence HHV-8 in early KS has not yet been shown. We used a mAb (LN53) against latent nuclear antigen-1 (LNA-1) encoded by ORF73 study distribution cell types latently infected patch, plaque, nodular KS, MCD, PEL. In present <10% cells forming walls ectatic vessels. surrounding slit-like vessels >90% spindle cells, but normal vascular endothelium. addition, colocalizes with endothelial growth factor receptor-3 (VEGFR-3), marker lymphatic precursor lesions, VEGFR-3 more extensively expressed than LNA-1, indicating that inducing proliferation VEGFR-3-positive endothelium directly. mantle zone large immunoblastic B cells. No staining for LNA-1 seen samples from multiple myeloma, prostate cancer, angiosarcoma, supporting absence any etiological link between these diseases HHV-8.