AIS is an oncogene amplified in squamous cell carcinoma

• DOI: 10.1073/pnas.97.10.5462
• Publication Date: 2002-07-26T14:35:07Z
• Authors: Kenji Hibi; Barry Trink; Meera Patturajan; William H. Westra; Otávia L. Caballero; David E. Hill; Edward A. Ratovitski; Jin Jen; David Sidransky
• Keywords: Lung Neoplasms; Gene Amplification; Chromosome Mapping; Mice, Nude; Nuclear Proteins; Oncogenes; Genes, p53; Phosphoproteins; Immunohistochemistry; Cell Line; 3. Good health; DNA-Binding Proteins; Alternative Splicing; Mice; 03 medical and health sciences; 0302 clinical medicine; Head and Neck Neoplasms; Carcinoma, Squamous Cell; Animals; Humans; Genes, Tumor Suppressor; Chromosomes, Human, Pair 3; In Situ Hybridization, Fluorescence

We and others recently isolated a human p53 homologue ( p40 / p51 p63 p73L ) localized the gene to distal long arm of chromosome 3. Here we sought examine role , two variants lacking N-terminal transactivation domain, in cancer. Fluorescent situ hybridization (FISH) analysis revealed frequent amplification this locus primary squamous cell carcinoma lung head neck cancer lines. (We named AIS for mplified i n s quamous carcinoma.) Furthermore, was accompanied by RNA protein overexpression variant p68 terminal domain. Protein tumors limited known harbor high frequency mutations. Overexpression Rat 1a cells led an increase soft agar growth tumor size mice. Our results support idea that plays oncogenic