Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling

• DOI: 10.1073/pnas.97.19.10424
• Publication Date: 2002-07-26T14:31:44Z
• Authors: James M. Angelastro; Lars Klimaschewski; Song Tang; Ottavio V. Vitolo; Tamily A. Weissman; Laura T. Donlin; Michael L. Shelanski; Lloyd A. Greene
• Keywords: 0301 basic medicine; Base Sequence; Tyrosine 3-Monooxygenase; Gene Expression Profiling; Cell Membrane; Proteins; PC12 Cells; Rats; 03 medical and health sciences; 14-3-3 Proteins; Gene Expression Regulation; Nerve Growth Factor; Animals; RNA, Messenger; Cell Division; Cytoskeleton; DNA Primers; Glutathione Transferase; Transcription Factors

Neurotrophic factors such as nerve growth factor (NGF) promote a wide variety of responses in neurons, including differentiation, survival, plasticity, and repair. Such actions often require changes gene expression. To identify the regulated genes thereby to more fully understand NGF mechanism, we carried out serial analysis expression (SAGE) profiling transcripts derived from rat PC12 cells before after NGF-promoted neuronal differentiation. Multiple criteria supported reliability profile. Approximately 157,000 SAGE tags were analyzed, representing at least 21,000 unique transcripts. Of these, nearly 800 by 6-fold or response NGF. 150 have been matched named genes, majority which not previously known be NGF-responsive. Functional categorization provides insight into complex, integrated mechanism promotes its multiple actions. It is anticipated that genomic sequence information accrues data here will continue provide about neurotrophic mechanisms.