Dermatan Sulfate Released after Injury Is a Potent Promoter of Fibroblast Growth Factor-2 Function

• DOI: 10.1074/jbc.273.43.28116
• Publication Date: 2002-07-26T15:02:22Z
• Authors: Stanley F. Penc; Bohdan Pomahac; Thomas Winkler; Robert A. Dorschner; Elof Eriksson; Mary Herndon; Richard L. Gallo
• Keywords: Dermatan sulfate; Versican; Perlecan; Syndecan 1; Iduronic acid

Proteoglycans have been shown <i>in vitro</i> to bind multiple components of the cellular microenvironment that function during wound healing. To study composition and these molecules when derived from an<i>in vivo</i> source, soluble proteoglycans released into human fluid were characterized evaluated for influence on fibroblast growth factor-2 activity. Immunoblot analysis revealed presence syndecan-1, syndecan-4, glypican, decorin, perlecan, versican. Sulfated glycosaminoglycan concentrations ranged 15 65 μg/ml, treatment with chondroitinase B showed a large proportion was dermatan sulfate. The total mixture present in supported ability signal cell proliferation. Dermatan sulfate, not heparan major contributor this activity, sulfate bound FGF-2 with<i>K</i> _d = 2.48 μm. These data demonstrate repair are functionally active provide first evidence is potent mediator responsiveness.