Identification of a Receptor for Reg (Regenerating Gene) Protein, a Pancreatic β-Cell Regeneration Factor

• DOI: 10.1074/jbc.275.15.10723
• Publication Date: 2002-07-26T14:46:49Z
• Authors: Seiichi Kobayashi; Takako Akiyama; Koji Nata; Michiaki Abe; Minori Tajima; Nausheen J. Shervani; Michiaki Unno; Seiki Matsuno; Hidetada Sasaki; Shin Takasawa; Hiroshi Okamoto
• Keywords: 0303 health sciences; DNA, Complementary; Calcium-Binding Proteins; Molecular Sequence Data; Nerve Tissue Proteins; Receptors, Cell Surface; CHO Cells; Rats; Islets of Langerhans; 03 medical and health sciences; Cricetinae; COS Cells; Lithostathine; Animals; Humans; Regeneration; Amino Acid Sequence

<i>Reg</i> (regeneratinggene) was isolated as a gene specifically expressed in regenerating islets (Terazono, K., Yamamoto, H., Takasawa, S., Shiga, Yonemura, Y., Tochino, and Okamoto, H. (1988) <i>J. Biol. Chem.</i> 263, 2111–2114). Rat human products, Reg/REG proteins, have been demonstrated to stimulate islet β-cell growth <i>in vitro</i> vivo</i> ameliorate experimental diabetes. In the present study, we cDNA for Reg protein receptor from rat library. The encoded cell surface 919-amino acid protein, cells into which had introduced bound with high affinity. When RINm5F cells, pancreatic line that shows Reg-dependent growth, transformants exhibited significant increases incorporation of 5′-bromo-2′-deoxyuridine well numbers response protein. A homology search revealed is homologue multiple exostoses-like gene, function has hitherto unknown. These results strongly suggest by mediates signal regeneration.