Role for Fes/Fps Tyrosine Kinase in Microtubule Nucleation through Its Fes/CIP4 Homology Domain

• DOI: 10.1074/jbc.c300289200
• Publication Date: 2003-11-26T20:08:23Z
• Authors: Shusuke Takahashi; Ryoko Inatome; Azusa Hotta; Qingyu Qin; Renee Hackenmiller; M. Celeste Simon; Hirohei Yamamura; Shigeru Yanagi
• Keywords: Microtubule nucleation

We have previously demonstrated that Fes/Fps (Fes) tyrosine kinase is involved in Semaphorin3A-mediated signaling. Here we report a role for Fes microtubule dynamics. A fibrous formation of was observed kinase-dependent manner, which associated with microtubules and functionally correlated bundling. Microtubule regeneration assays revealed aggregates colocalized gamma-tubulin at nucleation sites Fes/CIP4 homology (FCH) domain-dependent manner expression FCH domain-deleted mutants blocked normal centrosome formation. In support these observations, mouse embryonic fibroblasts derived from Fes-deficient mice displayed an aberrant structure unbundling disoriented filaments microtubules. Our findings suggest plays critical dynamics including bundling through its domain.