Structure-Expression Relationships of the 15-kDa Selenoprotein Gene

• DOI: 10.1074/jbc.m004014200
• Publication Date: 2002-07-26T15:05:53Z
• Authors: Easwari Kumaraswamy; Andrey Malykh; Konstantin V. Kor...; Sergei Kozyavkin; Yajun Hu; So Yeon Kwon; Mohamed E. Moustafa; Bradley A. Carlson; Marla J. Berry; Byeong J. Lee; Dolph L. Hatfield; Alan M. Diamond; Vadim N. Gladyshev
• Keywords: Selenoprotein; Selenocysteine; Selenoprotein P

Selenium has been implicated in cancer prevention, but the mechanism and possible involvement of selenoproteins this process are not understood. To elucidate whether 15-kDa selenoprotein may play a role etiology, complete sequence human protein gene was determined, various characteristics associated with expression were examined normal malignant cells tissues. The 51-kilobase pair for consisted five exons four introns localized on chromosome 1p31, genetic locus commonly mutated or deleted cancers. Two stem-loop structures resembling selenocysteine insertion elements identified 3′-untranslated region gene, only one these functional. alleles that differed by two single nucleotide polymorphic sites occurred within sequence-like structures. These polymorphisms resulted changes incorporation into responded differently to selenium supplementation. Human mouse genes manifested highest level prostate, liver, kidney, testis, brain, reduced substantially prostate cell line hepatocarcinoma. pattern tissues, occurrence expression, differential regulation polymorphisms, chromosomal location be relevant cancer.